Molecular identification of Diptera of forensic importance with the gene (COI Barcode), La Paz Bolivia
Abstract
One of the difficulties encountered is the correct identification of insects associated with cadaveric decomposition, which has led to the search and generation of new tools in molecular biology that facilitate the determination of specificities for the modification of the Post-Mortem Interval in an effective and accurate from immature stages; the collection and morphological taxonomy of Diptera were made in the first instance and then the Bar Code System (COI Barcode) was used for the molecular identification of problem insects by means of the mitochondrial COI gene in any phase of the biological cycle. Identifying three species of adults with a probability of 100% correspondence; the specimens: Sarconesia versicolor of the Calliphoridae Family and Fannia sp., were not found in the world databases of the GenBank and the Boldsystems, being necessary to update them by chronological succession patterns of cadaveric fauna in different geographical areas, whose practice would be applied in criminal investigations.
References
2. GilArriortua M., Saloña-Bordas M. I., & Pancorbo M. M. Uso de vestigios moleculares en entomología Forense. Ciencia Forense. 2015; 29–72.
3. Greenber B, Kunich JC (eds.). Entomology and the law: Flies as forensic Indicators. Cambridge University Press, Cambridge. 2002
4. Sakuma Calatayud, E. Caracterización de entomofauna cadavérica y tiempo de desarrollolarvario en la localidad de Mecapaca La Paz. La Paz Bolivia: tesis de grado. 2005.
5. Chávez Abasto Dorian Sandy, R. A. Investigación de fauna cadavérica de importancia forense y determinación del intervalo posmortem a través del estudio de muestras entomológicas En Cochabamba Bolivia. Rev.Inv.E Info Salud. 2008; 3(7):1-15.
6. Castillo Vega, P. A. Caracterización de la entomofauna asociada a cadáveres de cerdo (sus scrofa) fallecidos por lesiones producidas por heridas por arma blanca, en el municipio de Pucarani de la provincia Los Andes del departamento de la Paz-Bolivia, mayo a julio del 2013. Tesis, Universidad Mayor de San Andrés, La Paz, La Paz- Bolivia. 2013
7. Acho. Identificación de la entomofauna cadavérica asociada a la presencia de artrópodos en contexto de valle, localidad de millares municipio de Betanzos departamento de Potosí gestión 2008. Revista de Tecnología y Ciencias de la Salud. Junio 2014 Vol.1 No.1 48-65.
8. Solano J., Castro M. Identificación molecular de califóridos (Diptera: Calliphoridae) de importancia forense en Colombia. Revista Colombiana de Entomología. Julio - diciembre 2013; 39 (2): 281-290.
9. Wells JD, Stevens JR. Molecular methods for forensic entomology, pp. 437-452. Byrd JH,Castner JL (eds.). Forensic entomology: The utility of arthropods in legal investigations. CRC Press, Boca Raton. 2010.
10. Meiklejohn KA, Wallman JF, Dowton M. DNA-based identification of forensically important Australian Sarcophagidae (Diptera). International Journal of Legal Medicine. 2009; 125:27-32.
11. Barcode of life. What Is DNA Barcoding? 2017 Obtenido de http://www.barcodeoflife.org/content/about/what-dna-barcoding.
12. Miller, S., Dykes, D., & Polesky, H. A simple salting out procedure for extracting DNA from human cells. Nucleid Acid Research.1988; 16(3), 1215.
13. Hebert, P. D., Cywinska, A., Ball, S. L., & De Waard, J. R. Biological Identifications through DNA Barcodes. Proceedings of the Royal Society B, 270(1512).2003; 313–321. doi:10.1098/rspb.2002.2218,
14. Benson, D., Cavanaugh, M., Clark, K., Karsch-Mizrachi, I., Lipman, D., Ostell, J., & Sayers, E. GenBank. Nucleid Acids Research, 41, D36 - D42. 2013; doi:10.1093/nar/gks1195.
15. Ratnasingham, S. and Hebert, P.D.N. BOLD: The Barcode of Life Data System (http://www.barcodinglife.org). Molecular Ecology Notes.2007; 7, 355-364.
16. Zhuang Q., Cai J., Zhang M., Feng H, Guo Y. and Lan L. Molecular indentification of forensically significant beetles (Coleoptera) in China Based on COI gene. Revista Colombianade Entomol. 2011; gia 37: 95-102.
17. Pancorbo M. de, Ramos R., Saloña M., Sanchez P. Entomología molecular forense. Ciencia Forense.2006; 8:107-130.
18. Boehme P., Amendt J., Zehner R. The use of CoI barcodes for molecular identification of forensically important fly species in Germany. Parasitology Research. 2012; 110: 2325-2332.
19. Hofer, I.M.S., Hart, A.J., Matin-Vega, D. & Hall, M. J. R. Optimising crime scene temperature collection for forensic entomology casework. Forensic Sci Int. 2017 Jan;270:129-138. doi: 10.1016/j.forsciint.2016.11.019. Epub 2016 Nov 17.
20. Morlais I, Severson DW. Complete mitochondrial DNA sequence and amino acid analysis of the cytochrome c oxidase subunit I (COI) from Aedes aegypti. DNA sequence. 2002; 13 (2):123-127.
21. Wells JD, Wall R, Stevens JR. Phylogenetic analysis of forensically important Luciliaflies based on cytochrome oxidase I sequence: A cautionary tale for forensic species determination. International Journal of Legal Medicine.2007; 121:229-233.
The intellectual property of accepted works belongs to their authors, however access to them is totally open and free, so they can be reproduced totally or partially with the only limitation of recognizing the authorship and the source of publication (Revista Medicina Legal de Costa Rica), as long as said exploitation does not have commercial character.
